Original Article

Effect of Vitamin A and E on the Interaction Between 2-Acetylaminofluorene and Rat Liver DNA in Vivo

Young-Sook Hong
Author Information & Copyright
Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul-120, Korea, Korea.
Corresponding author: Young-Sook Hong. Department of Biochemistry, College of Medicine Ewha Woman's University, Seoul-120, Korea.

Copyright ⓒ 1985. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

Binding of the hepatocarcinogen 2-acetylaminofluorene(AAF) to the DNA of rat liver homogenates was examined in vivo. Animals fed low and high concentrations vitamin A and E for 28 days and DNA isolated 2 hr following a single i.p. injection of[9-14C]acetylaminofluorene. The precipitated DNA was dissol-ved in MUP, blended and passed through an HAP column. UV-absorbing material eluted with 0.48M Nap. Following dialysis, the fraction was enzymatically hydrolyzed purified by Sephadex LH-20 chromatography and the adducts were separated by HPLC. The ability of vitamin A and E on the binding of AAF to liver DNA in vivo was markedly inhibited the formation of the proximate carcinogen, N-(deoxyguanosine-N2-yl) AAF(C-8 adduct). Vitamin A at higher concentration(2,000I.U.)exerted a stronger inhibitory effect on the formation C-8 adducts. A similar inhibition pattern was observed in higher concentration vitamin E administered rats. The data presented here demonstrated marked inhibits in the pattern of C-8 deoxyguanosine adduct formation during the premaligment phase of 2-AAF carcinogenesis.