The Ewha Medical Journal
Ewha Womans University School of Medicine
Original Article

Effect of Various Irrigation Fluids of Arterial and Venous Endothelium after Ischemia

Chin Ho Yoon, Han Joong Kim

Copyright ⓒ 1991. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

This study examines the extent of endothelial damage following a period of irrigation with various crystalloid irrigation fluds. Both arteries and veins were evaluated after irrigation with normal saline, lactated Ringer's. balanced salt solution (BSS), and balanced salt solution plus(BSS+). The arterial and venous endothelia were examined with the scanning electron microscope. Using a randomized blind observer scoring system. micrographs were evaluated for changes in nuclear shape, cell junction integrity, cytoplasm changes, and sloughing of the endothelial cell layer. BSS+ and BSS produced stastically significant(p<.001) improvement over the other two irrigation fluids in the arteries. BSS+ and BSS were stastically superior(P<.00l) in the venous vessels. The compositions of BSS and BSS+ tend to maintain a physiologic environment in the presence of ischemia. These maintained a morphologic appearance closer to that of perfusion-fixed controls The data suggest a protective effect of such physiologic preparations on the endothelium. The preservation of intact endothelium may play a role in decreased platelet activation. continued production of prostacyclin, and maintenance of and intact barrier between the intra-cellular and extracellular spaces. This could enhance the survival of transplanted or transferred tissue, by helping to maintain nearly normal endothelium during surgery.