Study of M.Tuberculosis and Non-Tuberculous Mycobacterium in Subacute Necrotizing Lymphadenitis Using Multiplex PCR

Min-Sun Cho, Woon Sup Han

Copyright ⓒ 2003. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jun 30, 2003

Abstract

Objectives

Subacute necrotizing lymphadenitis is a self-limiting disorder that typically affects cervical lymph nodes of young women. Although autoimmune reaction and a viral etiology have been suggested, the definite cause has been uncertain. We evaluated the association of M. tuberculosis and non-tuberculous mycobacterium with this disorder.

Materials and Methods

From 1994 to 2002, Clinicopathological findings of 38 cases were reviewed. Multiplex nested PCR was done using DNA extracted from paraffin-embedded tissue. Sequencing analysis was done to the cases showing positive band.

Results

3 cases among 38(7.89%) showed amplified non-tuberculous mycobacterium DNA. Two cases are M. gordonae and one is M. fortuitum by sequencing analysis. All cases revealed no positive band for M. tuberculosis. Negative control cases showed no bands for both mycobacteriums.

Conclusion

Although 7.89% of cases showed amplified non-tuberculous mycobacterium DNA, more study is needed to confirm the association of non-tuberculous mycobacterium with subacute necrotizing lymphadenitis.

Keywords: Subacute necrotizing lymphadenitis; Tuberculosis; Nontuberculous mycobacterium; Multiplex PCR