The Ewha Medical Journal
Ewha Womans University School of Medicine
Original Article

The Effect of Ovarian Hormone on the Phosphatase Activity in the Ovariectomized Rat Uterus

Sung Rye Kim
Corresponding author: Sung Rye Kim. College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1980. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

Quantitative analysis of the activities of phosphatase in ovariectomized rat uterus was carried out.

1. The activities of total phosphatase were decreased by ovarian steroids (except progesterone treated group at pH 9).

2. The ratio of acid: alkali, neutral phosphatase was 4 : 1.

3. Among total enzymes, the proportion of Mg++ - dependent phosphatase was much higher than the other specific enzyme.

4. The activities of alkali, acid phosphatase were decreased by cystein, but the activity of neutral phosphatase was increased.

5. At the neutral media, the experimental group treated with progesterone and estradiol-17β on day 1 appeared the following special figures.

(1) The activity of total phosphatase was low.

(2) K+ - dependent phosphatase was negligible.

(3) Mg++ - dependent phosphatase transformed into Mg++ - independent phosphatase.

(4) The activity of phosphatase was stimulated by cystein.

6. These results means the fact that phosphatase activity is affected by ovarian steroids, and by pH.