The Ewha Medical Journal
Ewha Womans University School of Medicine
Original Article

Studies on the Hepatic Microsomal Mixed Function Oxidase System with Higenamine on the Rat

Young Sook Hong, Young Sook Pae*, Nak Eung Sung
Corresponding author: Nak-Eung Sung. Department of Pharmacology, College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1985. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

The effect of intraperitonelly administered higenamine on the cytochrome P -450, b5, P-nitroanisole-O-demethylase and lipid peroxidation in the rat hepatic microsomes were determined. The following results were obtained ; 1) The intraperitonelly administered higenamine increased contents of rat hepatic microsomal cytochrome P-450 and b5. 2) The principal role of cytochrome P-450 in mixed function oxidase reactions includes hydroxylation of various drugs, fatty acids, steroids, pesticides and carcinogens. The increased cytochrome P-450 induced by higenamine decreased on the both AAF of N-hydorxylation and Ring-hydroxylation. 3) Activity of P-nitroanisole-O-demethylase in hepatic microsomes with higenamine was decreased accorting to incresed dosage of higenamine. 4) The formation of lipid peroxides was increased according to increased dosage of higenamine.