The Ewha Medical Journal
Ewha Womans University School of Medicine
Original Article

Molecular Cloning of Novel Clathrin Assembly Protein Gene from Rat Brain

Hyung-Lae Kim, Young Sook Hong

Copyright ⓒ 1996. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

Binding of clathrin assembly protein to clathrin triskelia induces their assembly into clathrin-coated vesicle in neurons. The clathrin binding protein is a neuronal-specific, synapse associated protein that is expressed nonuniformly in rat brain. We isolated two cDNAs, encoding the novel clathrin assembly protein, which has a 73% amino acid homology compared with that of AP180 protein when translated into amino acids. The deduced molecular weight is 64kD. The N-terminal domain harbouring clathrin binding site is very similar to that of AP180, and the C-terminal domain is much more different with that of AP180, which suggests that the novel protein mediates the assembly of clathrin and its regulatory role in the release of secretory vesicle.

Keywords: Clathrin assembly protein; Clathrin-coated vesicle; Gene cloning; AP180; AP-3