The Ewha Medical Journal
Ewha Womans University School of Medicine
Original Article

CD23(FcεRII) Expression on Splenic B Cells in Paragonimus Westermani-infected Mice

Myeong Heon Shin

Copyright ⓒ 1996. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

Object

Elevation of serum lgE is the most characteristic immune response in helminthic infections. Recently, expression of CD23(FcεRII on B lymphocytes play a major role in lgE production in allergic diseases. However, the mechanisms causing increased lgE production during helminthic infections are poorly understood. In the present study, the expression of CD 23 on splenic B lymphocytes during the course of infection with Paragonimus westermani was examined.

Methods

Female, 4-6-week old BALB/c mice were inoculated orally with 20 metacercariae of P. westermani. For detection of CD23 positive B lymphocytes by flow cytometry, splenocytes from infected mice and non-infected age matched controls were stained with FITC-conjugatedrat anti-mouse DB23 and PE-conjugated rat anti-mouse CD45R/B220 mono-clonal antibody. And also, to observe the effect of metacercarial ESP on the expression of CD23 antigen, splenocytes from non-infected mice were incubated in the presence of ESP at 37℃ for 6 h and 24 h.

Results

The frequency of CD23 positive B lymphocytes of infected mice was increased significantly(p<0.05) at two and three weeks after infection(43.4±7.52% and 44.4±2.99%, respectively) and persisted the higher levels at four and six weeks after infection. Expression of CD23 antigen of cultured splenocytes from non-infected mice in the presence of metacercarial ESP was increased significantly(p<0.05) at 24 h after incubation(60.1±7.54%).

Conclusion

These results suggest that the expression of CD23 antigen induced by metacercarial ESP might play a important role in serum lgE production in mice infected with P. westermani.

Keywords: CD23(FcεRII); Excretory-secretory products(ESP); Paragonimus westermani