The Ewha Medical Journal
Ewha Womans University School of Medicine
Original Article

Influence of Diazepam, Cimetidine and Sulpiride on the Level of Hepatic Microsomal Cytochrome P450 in Rats

B.H. Kim, Y.A. Ham, Y.S. Hong, N.E. Sung
Corresponding author: N.E.Sung. Department of Biochemistry, College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1979. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015


Cytochrome P-450 is a terminal oxidase of the hepatic microsomal monooxygenase system associated with the oxidative biotransformation of a varity of lipophilic endogenous and exogenous compounds, and generally is assayed by CO-binding spectro-photometry of dithionite-reduced samples. In well fed normal male rats with or without stress condition, the dffect of antiulcer drug; cimetidine, sulpiride and diazepam on the level of microsomal cytochrome P-450 were determined. Only stress condition produced on significant effect in cytochrome P-450 contents. Administration of cimetidine, sulpiride and diazepam in this condition caused a 44.1%~87.5% increment in cytochrome P-450, diazepam produced the most increase. After diazepam treatment of rats, the peak position of cytochrome P-450 shifted to a longer wave length of 452 nm.