The Ewha Medical Journal

"Rapid urease test"

Original Articles

[English]

The Clinical Value of Rapid Urease Test(CLO Test) for the Detection of Helicobacter Pylori in Gastric Biopsy Specimens of Duodenal Ulcer Patients: Doe Young Kim, Sung Sook Kim; Ihwa Ŭidae chi 1995;18(4):375-381. Published online July 24, 2015; DOI: https://doi.org/10.12771/emj.1995.18.4.375

Abstract
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Objectives

This study was aimed to assess the value of rapid urease test (CLO test) for thedetection of Helicobacter (H.) pylori in patiens with duodenal ulcer and compare its resultwith histological technique using H & E stain.

Methods

The CLO test and histological study were done using three pieces of antral biopsyspecimens in 46 patients with duodenal ulcer. The clinical histories such as smoking, bloodtype or the previous use of NSAIDs were obtained from the patients.

Results

1) The CLO test was positive in 35 (76.1%) among 46 patients with duodenal ulcer, while H & E stain was positive in 91.3%.

2) When H & E stain positive case was diagnosed as H. pylori infection, the sensitivity of CLO test was 81.0%, the specificitry 75.0% and the predictability was 97.1%.

3) The grading of gastric inflammation showed that the higher the histologogical grade, the more likely CLO test would be positive.

4) There were no differences of recurring history of duodenal ulcer, smoking rate, NSAIDs history and blood type between 34 cases of both CLO and H & E positive cases and 3 cases ofboth negative cases except a mild increasing tendency of age, male predominance and scarringstage of duodenal ulcer in both negative cases.

Conclusion

The CLO test is a rapid and simple test, but it should require other complementary diagnostic tests to increase the sensitivity and specificity.

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[English]

The Comparison of HP™ Test, a New Domestically Manufactured Rapid Urease Test, with CLO™ Test: Jeong Yoon Yim, Young Mee Ahn, Doe Young Kim, Il Whan Moon; Ihwa Ŭidae chi 1999;22(4):213-217. Published online December 31, 1999; DOI: https://doi.org/10.12771/emj.1999.22.4.213

Abstract
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Although CLO test is one of the most commonly used rapid urease test for the diagnosis of Helicobacter(H.) pylori infection. Recently a Korean company, Jongkeundang, has succeeded in manufacturing the rapid urease test and named it as HP™ test. The advantages of HP test are to have two agar gels in a plate and react faster in despite of same cost with CLO test. We performed this study to compare the results of the HP test with those of the CLO test and know if there is any advantage of using two biopsy samples instead of using only one sample.

One hundred and eight patients underwent gastroscopy and two biopsy specimens from the greater curvature of the antrum was used for HP test and CLO test. Another biopsy specimen from lesser curvature of midbody was used for HP test. We read them at 20min, 1hour, 3hours, and 24hours.

The positive rate of HP test in antrum was 49.1%(53/108) which was the same as CLO test in antrum. The concordance rate of the results of two tests in the same site(antrum) were 98.2%. In terms of a time change to a positive test, both tests were not significantly different with each other. ; 3hour positive rate of HP test was 94.4% and that of HP test was 92.5%. Four patients(5.6%) performed HP test had positive results after 2hours known final reading time.

The positive rate of HP test in body was 52.8%(57/108) and that of HP test in antrum or body was 53.7%(58/108). The concordance rate of the results of antrum and body in HP test was 94.4%. Five of the negative HP test in antrum(4.7%) had positive results in gastric body. They were negative CLO test in antrum.

In conclusion, HP test was thought to be as valuable as CLO test for the diagnosis of H. pylori infection and has a merit of decreasing sampling error by using two samples, even though it is a little. However, It had not quicker time to positivity than CLO test and might have false negative results if it were read at 2hours after reaction. So, Final reading time should be reevaluated.