Abstract

• Quantitative analysis of the activities of phosphatase in ovariectomized rat uterus was carried out.
  1. The activities of total phosphatase were decreased by ovarian steroids (except progesterone treated group at pH 9).
  2. The ratio of acid: alkali, neutral phosphatase was 4 : 1.
  3. Among total enzymes, the proportion of Mg⁺⁺ - dependent phosphatase was much higher than the other specific enzyme.
  4. The activities of alkali, acid phosphatase were decreased by cystein, but the activity of neutral phosphatase was increased.
  5. At the neutral media, the experimental group treated with progesterone and estradiol-17β on day 1 appeared the following special figures.
  (1) The activity of total phosphatase was low.
  (2) K⁺ - dependent phosphatase was negligible.
  (3) Mg⁺⁺ - dependent phosphatase transformed into Mg⁺⁺ - independent phosphatase.
  (4) The activity of phosphatase was stimulated by cystein.
  6. These results means the fact that phosphatase activity is affected by ovarian steroids, and by pH.