Original Article

Fetal Cord Serum Supplemented Ham's F-10 vs. Medi-Cult for in Vitro Fertilization Culture Medium

Hye Won Chung
Author Information & Copyright
Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1996. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

The media for assisted fertilization from Medi-Cult is based on a Synthetic Serum Replacement(SSR). The main principle of SSR is a metal iron buffer containing a balanced mixture of iron and trace metals. Recently, Synthetic Serum Supplement(SSS) and Medi-cult are commonly used.

These synthetic substitutes have some advandages, such as safty from viral contamination and no needs of bioassay.

To compare the usefulness between the Medi-cult and Ham's F-10 supplemented with fetal cord serum for the culture medium of in vitro fertilization, fertilization rate, cleavage rate and pregnancy rate were studied. It is concluded that Medi-cult was better than Ham's F-10 supplemented with fetal cord serum according to fertilization rate, cleavage rate, pregnancy rate and ongoing pregnancy, but that differences did not have statistical significance.