Original Article

Relationship between the Stimulated Peak Growth Hormone Level and Metabolic Parameters in Children with Growth Hormone Deficiency

Seong Yong Lee 1 , * https://orcid.org/0000-0002-7077-869X
Author Information & Copyright
1Department of Pediatrics, SMG-SNU Boramae Medical Center, Seoul, Korea
*Corresponding author: Seong Yong Lee, Department of Pediatrics, SMG-SNU Boramae Medical Center, 20 Boramae-Ro 5-Gil, Dongjak-Gu, Seoul 07061, Korea, Tel: 82-2-870-2363, Fax: 82-2-870-3363, E-mail: gnoygnoes@hanmail.net

© Copyright 2023 Ewha Womans University College of Medicine and Ewha Medical Research Institute. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Nov 30, 2022; Revised: Dec 30, 2022; Accepted: Jan 16, 2023

Published Online: Jan 31, 2023

ABSTRACT

Objectives:

This study investigated the relationship between the stimulated peak growth hormone (PGH) level and comprehensive metabolic markers for glucose and lipid metabolism, and liver steatosis in prepubertal children with GH deficiency (GHD).

Methods:

Sixty-nine prepubertal children with GHD were divided into overweight/obesity (body mass index [BMI]≥85th percentile) and normal BMI groups. The associations between PGH level and metabolic parameters including homeostatic model assessment-insulin resistance (HOMA-IR), lipid profiles, AST, and ALT were evaluated.

Results:

The LDL cholesterol level was significantly higher in the overweight/obesity group than in the normal BMI group. PGH level was negatively associated with the BMI SD score (SDS) (r=−0.26, P=0.029) and ALT (r=−0.36, P=0.004) levels, whereas it was positively associated with the HDL-cholesterol (HDL-C) level (r=0.38, P=0.002). In multivariate analyses, PGH level was positively associated with HDL-C level (P=0.002) and negatively associated with ALT level (P=0.028) after adjusting for age, sex, BMI SDS, HOMA-IR, and TG level.

Conclusion:

PGH level in pre-pubertal children with GHD was positively and negatively associated with HDL-C and ALT, respectively, even if they were within normal range, regardless of BMI.

Keywords: Child; Growth hormone; Metabolism

Introduction

In children with short stature, growth hormone (GH) deficiency is characterized by a sub-normal increase in GH level after pharmacological stimulation. In general, a stimulated peak GH (PGH) level<10 ng/mL in two GH stimulation tests confirms GH deficiency (GHD). However, the GH stimulation test is a non-physiological test with a poor reproducibility and a high incidence of false-positive results [1,2].

The GH response to pharmacological stimulation varies with the stimulant type, state of GH secretion preceding the stimulus, age, puberty, and other physiological conditions [3]. Body mass index (BMI) also affects the stimulated PGH level [47].

The spontaneous and stimulated GH levels are lower in obese children [8,9] and adults [10,11] than in normal controls. Therefore, the possibility of the over-diagnosis of GHD in obese people has been raised [12].

Despite these limitations, GH stimulation tests have been widely used as the most useful method to diagnose GHD.

GH is the main promoter of skeletal growth and has several metabolic functions, such as the regulation of body composition, protein synthesis, and glucose and lipid metabolism [13,14].

Previous studies have found that obesity and metabolic derangements are related to GHD in adults [1518]. Obesity and metabolic syndrome are common in patients with adult-onset GHD and adolescents with childhood-onset GHD who discontinued GH treatment after epiphyseal closure [1921].

Although several studies of children with GHD have shown a reciprocal relationship between the stimulated PGH level and BMI, only a few studies have investigated the relationships between the stimulated PGH level and metabolic parameters in children with GHD [2224]. In most of these studies, metabolic markers are limited to a certain type of metabolic disturbance, and the participants’ age varied, mostly including adolescents during and after puberty.

The present study investigated the relationship between the stimulated PGH level and comprehensive metabolic markers, including insulin resistance index, lipid profiles, AST, and ALT levels in prepubertal children with GHD.

Methods

1. Study participants

The medical records of 141 children who underwent GH stimulation tests between June 2018 and August 2022 at a single hospital were retrospectively reviewed. Sixty children with a PGH level≥10 ng/mL after GH stimulation were excluded. In total, 81 children were diagnosed with GHD (PGH level<10 ng/mL).

None of the patients had multiple pituitary hormone deficiencies or chronic diseases. After excluding three adolescents undergoing puberty, two children with congenital abnormalities (one each with Leri Weill disease and congenital heart disease), four children with hyperlipidemia with a family history, and three patients with incomplete data, 69 GHD patients were included (Fig. 1).

emj-46-1-1-g1
Fig. 1. Flowchart of the study participants. GH, growth hormone; GHD, GH deficiency; BMI, body mass index.
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Children with isolated GHD with no other pituitary hormone deficiency were included in this study, even if they had brain MRI abnormalities. These included mild anterior pituitary hypoplasia, Rathke cleft cyst, arachnoid cyst, pineal cyst, and septum cavum pellucidum in 26, 10, 1, 1, and 1 patient, respectively.

2. Methods

Height was measured using a Harpenden Stadiometer (Holtain, Crymych, UK) and recorded to the nearest 0.1 cm. Weight was measured using an electronic scale (CAS, Seoul, Korea) and recorded to the nearest 0.1 kg. BMI was calculated as weight (kg) divided by height squared (m2). The SD scores (SDS) for height, weight, and BMI were calculated using the 2017 Korean National Growth Chart [25]. Bone age was calculated using the Greulich-Pyle method.

GH stimulation tests were performed after an overnight fast using two stimulants, including insulin, L-dopa, and arginine (insulin/L-dopa, insulin/arginine, and L-dopa/arginine in 5, 1, and 63 patients, respectively).

3. Measurements

After 12 hr of fasting, the lipid profile and insulin-like growth factor 1 (IGF1), insulin, glucose, AST and ALT levels were measured according to the GH test protocol of SMG-SNU Boramae Medical center.

The IGF1, GH, and insulin levels were measured using immunoradiometric assays (IRMAs) (IGF1 IRMA Kit: Immunotech S.R.O., Prague, Czech Republic; GH IRMA Kit: Institute of isotopes, Budapest, Hungary; Insulin IRMA Kit: DIAsource ImmunoAssay, Louvain-la-Neuve, Belgium). The total cholesterol (TC), HDL-cholesterol (HDL-C), TG, and glucose levels were analyzed using standard enzymatic methods (Cobas 8000 c702; Roche, Rotkreuz, Switzerland).

AST and ALT levels were measured using absorbance assay (Cobas 8000 c702; Roche).

The LDL-cholesterol (LDL-C) level was determined using the Friedewald equation: LDL-C=TC−HDL-C−TG/5 [26].

The insulin resistance index was calculated as: homeostatic model assessment-insulin resistance (HOMA-IR)=(glucose [mg/dL]×insulin [IU/L]/405) [27].

4. Statistical analysis

Statistical analyses were performed using SPSS software (version 27.0; IBM, Armonk, NY, USA). Variables are presented as means±SD or medians and interquartile ranges. The nonparametric Wilcoxon Rank-Sum test was used to compare variables between the groups. Pearson correlation analysis was used to determine the correlations of PGH level with clinical and metabolic parameters. Linear regression analyses were performed to evaluate the relationships between metabolic parameters and PGH level. A multivariate regression model was constructed based on the variables with P<0.05 in the univariate analysis and possible covariates such as age, sex, and BMI SD score. P<0.05 was considered statistically significant.

Results

1. Clinical and laboratory characteristics of the participants

Table 1 presents the clinical and laboratory characteristics of the study participants.

Table 1. Clinical and laboratory characteristics of the participants
Mean±SD Median Interquartile range
Age (yr) 8.35±2.61 8.58 5.95, 10.65
Bone age (yr) 7.00±2.57 7.50 5.00, 9.50
Height (cm) 116.57±13.87 119.50 105.50, 128.30
Height SDS –2.34±0.44 –2.23 –2.46, –2.07
Weight (kg) 23.69±8.19 22.30 17.20, 28.40
Weight SDS –1.51±0.86 –1.51 –2.14, –1.01
BMI (kg/m2) 16.90±2.50 16.34 15.02, 18.20
BMI SDS –0.28±1.00 –0.33 –0.82, 0.20
PGH-T (ng/mL) 6.36±2.23 6.73 4.78, 8.38
PGH-L (ng/mL) 5.08±1.00 4.78 3.21, 6.78
PGH-A (ng/mL) 5.02±2.63 5.04 2.61, 7.13
IGF1 (ng/mL) 190.60±81.96 177.60 130.90, 227.00
Insulin (mIU/L) 8.36±3.32 8.65 5.40, 10.12
Glucose (mg/dL) 87.39±5.73 87.00 83.00, 92.00
HOMA-IR 1.82±0.77 1.80 1.17, 2.34
Total cholesterol (mg/dL) 167.43±31.48 168.00 148.00, 185.00
Triglyceride (mg/dL) 86.58±45.73 76.00 54.00, 109.00
HDL-C (mg/dL) 60.20±10.71 60.00 54.00, 66.00
LDL-C (mg/dL) 92.13±20.82 92.00 79.00, 103.00
AST (IU/L) 28.09±5.37 27.50 24.00, 32.00
ALT (IU/L) 13.67±6.34 12.00 10.00, 14.00

Bone age was evaluated using the Greulich-Pyle method.

SDS, SD score; BMI, body mass index; PGH-T, peak growth hormone level determined by two stimulation tests; PGH-L, peak growth hormone level stimulated by L-dopa; PGH-A, peak growth hormone level stimulated by arginine; IGF1, insulin-like growth factor 1; HOMA-IR, homeostatic model assessment-insulin resistance; HDL-C, HDL-cholesterol; LDL-C, LDL-cholesterol.

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Of the 69 GHD children, 42 (60.1%) were males and 27 (39.1 %) were females; 3 (4.3%) children were obese (BMI≥95th percentile) and 5 (7.2%) were overweight (85th≤BMI<95th percentile). In total, 8 (11.6%) children with overweight or obesity were included in the overweight/obesity group.

None of the children had an abnormally high glucose level (>100 mg/dL), whereas 3 children (4.3%) had high insulin levels (>15 mIU/L) and 6 children (8.7%) had a HOMA-IR>3.

Hypercholesterolemia (TC>200 mg/dL and/or LDL-C>130 mg/dL) was present in 7 (10.1%) children. Hypertriglyceridemia (TG>150 mg/dL) and low HDL-C (<40 mg/dL) were present in 4 (5.8%) and 2 (2.9%) children, respectively. In total, 10 (14.5%) children had dyslipidemia.

None of the study participants had a high AST (>40 IU/L) or ALT (>40 IU/L) level. One patient had an AST level of 40 IU/mL, and another had an ALT level of 40 IU/mL.

2. Comparison of the overweight/obesity and normal groups

The height, weight, and BMI were higher in the overweight/obesity group than in the normal group (Table 2). The PGH level determined by two stimulation tests (PGH-T), PGH level stimulated by L-dopa (PGH-L) and PGH level stimulated by arginine (PGH-A) were lower in the overweight/obesity group than in the normal group, albeit without statistical significance (PGH-T, 5.22 vs. 7.01 ng/mL, P=0.139; PGH-L, 4.20 vs. 5.12 ng/mL, P=0.261; PGH-A, 4.50 vs. 5.05 ng/mL, P=0.543).

Table 2. Comparison of clinical and laboratory parameters between overweight/obesity and normal group
Normal group (n=61) Overweight/obesity group (n=8) P-value
Age (yr) 9.17 (6.31, 10.74) 6.42 (5.08, 8.69) 0.139
Bone age (yr) 7.5 (5.00, 9.50) 5.0 (3.69, 7.63) 0.165
Height SDS –2.26 (–2.58, –2.10) –2.06 (–2.18, –1.99) 0.038
Weight SDS –1.63 (–2.23, –1.22) –0.28 (–0.39, 0.30) <0.001
BMI SDS –0.46 (–1.04, –0.01) 1.54 (1.16, 2.09) <0.001
PGH-T (ng/mL) 7.01 (4.87, 8.49) 5.22 (3.94, 6.72) 0.139
PGH-L (ng/mL) 5.12 (3.21, 6.94) 4.20 (2.97, 4.93) 0.261
PGH-A (ng/mL) 5.05 (2.73, 7.44) 4.80 (2.43, 5.89) 0.543
IGF1 (ng/mL) 178.2 (130.90, 229.80) 165.8 (142.68, 216.83) 0.613
Insulin (mIU/L) 8.55 (5.40, 10.10) 10.15 (7.95, 12.18) 0.121
Glucose (mg/dL) 87 (83.0, 92.0) 88 (87.5, 90.8) 0.436
HOMA-IR 1.75 (1.15, 2.16) 2.21 (1.81, 2.73) 0.094
Total cholesterol (mg/dL) 167 (147.0, 185.0) 182 (167.3, 195.3) 0.076
Triglyceride (mg/dL) 72 (54.0, 99.0) 103 (73.5, 144.0) 0.077
HDL-C (mg/dL) 60 (55.0, 66.0) 54 (50.5, 60.0) 0.058
LDL-C (mg/dL) 89.0 (77.0, 102.0) 100.5 (91.5, 113.5) 0.044
AST (IU/L) 27 (24.0, 32.0) 29 (26.0, 34.5) 0.410
ALT (IU/L) 12.0 (10.0, 14.0) 13.5 (11.8, 21.0) 0.081

Data are expressed as median and interquartile range.

Bone age was evaluated using the Greulich-Pyle method.

SDS, SD score; BMI, body mass index; PGH-T, peak growth hormone level determined by two stimulation tests; PGH-L, peak growth hormone level stimulated by L-dopa; PGH-A, peak growth hormone level stimulated by arginine; IGF1, insulin-like growth factor 1; HOMA-IR, homeostatic model assessment-insulin resistance; HDL-C, HDL-cholesterol; LDL-C, LDL-cholesterol.

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The HOMA-IR, TC, TG, and ALT levels were higher and HDL-C level was also lower in the overweight/obesity group than in the normal group, but there was no statistical significance. The LDL-C level was significantly higher in the overweight/obesity group than in the normal group (P=0.044).

3. Relationships between the PGH level and metabolic parameters

Correlation analysis showed that PGH-T level was negatively associated with BMI SDS (r=−0.26, P=0.029). The PGH-T was positively associated with the HDL-C level (r=0.38, P=0.002) and negatively associated with the ALT level (r=−0.36, P=0.004) (Fig. 2). In a partial correlation analysis controlled by BMI SDS, PGH-T level was also positively and negatively associated with the HDL-C (r=0.34, P=0.005) and ALT (r=−0.29, P=0.021) levels, respectively.

emj-46-1-1-g2
Fig. 2. Correlation between PGH-T and BMI SDS (A), HDL-C (B) and ALT (C) levels. PGH-T, peak growth hormone level determined by two provocation tests; BMI, body mass index; SDS, SD score; HDL-C, HDL-cholesterol.
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The PGH-L was also negatively associated with, BMI SDS (r=−0.36, P=0.003) and the ALT level (r=-0.25, P=0.049) (Fig. 3). However, PGH-L was not associated with the ALT level controlled by BMI SDS (r=−0.14, P=0.267).

emj-46-1-1-g3
Fig. 3. Correlation between PGH-L and BMI SDS (A), HDL-C (B) and ALT (C) levels. PGH-L, peak growth hormone level stimulated by L-dopa; BMI, body mass index; SDS, SD score; HDL-C, HDL-cholesterol.
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The PGH-A was not associated with BMI SDS but was positively and negatively associated with the HDL-C (r=0.41, P=0.001) and ALT (r=−0.32, P=0.012) levels, respectively (Fig. 4).

emj-46-1-1-g4
Fig. 4. Correlation between PGH-A and BMI SDS (A), HDL-C (B) and ALT (C) levels. PGH-A, peak growth hormone level stimulated by arginine; BMI, body mass index; SDS, SD score; HDL-C, HDL-cholesterol.
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Fasting insulin, glucose levels, and HOMA-IR were not correlated with the PGH-T, PGH-L, and PGH-A levels (Table 3).

Table 3. Correlation between peak growth hormone level and clinical and laboratory parameters
PGH-T PGH-L PGH-A
r P-value r P-value r P-value
Age 0.14 0.261 0.09 0.454 0.02 0.890
Bone age 0.11 0.367 0.07 0.570 –0.01 0.940
Height SDS –0.03 0.784 –0.06 0.642 0.01 0.933
Weight SDS –0.22 0.079 –0.32 0.007 –0.04 0.748
BMI SDS –0.26 0.029 –0.36 0.003 –0.09 0.488
IGF1 0.13 0.270 0.05 0.663 0.20 0.117
Insulin –0.004 0.977 –0.03 0.814 –0.03 0.803
Glucose –0.02 0.902 –0.12 0.324 0.05 0.705
HOMA-IR –0.01 0.957 –0.06 0.644 –0.02 0.862
Total cholesterol 0.22 0.075 0.08 0.528 0.2 0.074
Triglyceride –0.22 0.073 –0.18 0.133 –0.19 0.137
HDL-C 0.38 0.002 0.20 0.098 0.41 0.001
LDL-C 0.11 0.369 0.0045 0.971 0.17 0.181
AST 0.01 0.912 –0.08 0.541 0.14 0.282
ALT –0.36 0.004 –0.25 0.049 –0.32 0.012

PGH-T, peak growth hormone level determined by two stimulation tests; PGH-L, peak growth hormone level stimulated by L-dopa; PGH-A, peak growth hormone level stimulated by arginine; SDS, SD score; BMI, body mass index; IGF1, insulin-like growth factor 1; Ln IGF1, log-transformed insulin-like growth factor 1; HOMA-IR, homeostatic model assessment-insulin resistance; HDL-C, HDL-cholesterol; LDL-C, LDL-cholesterol.

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4. Linear regression analyses with HDL-C and ALT levels as dependent variables

Tables 4 and 5 present the results of univariate and multivariate linear regression analyses with HDL-C and ALT levels as dependent variables, respectively.

Table 4. Factors associated with HDL cholesterol levels
Univariate Multivariate
Total L-dopa Arginine
β P-value β P-value β P-value β P-value
Age 0.456 0.363 0.14 0.198 0.205 0.074 0.182 0.091
Sex 0.762 0.775 0.005 0.965 –0.03 0.793 –0.049 0.644
BMI SDS –0.068 0.140 0.053 0.635 –0.016 0.891 –0.012 0.915
PGH-T 1.722 0.001 1.534 0.002 -
PGH-L 0.924 0.098 0.133 0.243
PGH-A 1.631 0.001 1.322 0.003
HOMA-IR –2.388 0.166 –0.047 0.673 –0.029 0.809 –0.040 0.720
Triglyceride –0.116 0.000 –0.092 <0.001 –0.111 <0.001 –0.098 <0.001
ALT –0.478 0.021 0.073 0.571 –0.044 0.741 0.062 0.636

BMI, body mass index; SDS, SD score; PGH-T, peak growth hormone level determined by two stimulation tests; PGH-L, peak growth hormone level stimulated by L-dopa; PGH-A, peak growth hormone level stimulated by arginine; HOMA-IR, homeostatic model assessment-insulin resistance.

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Table 5. Factors associated with ALT levels
Univariate Multivariate
Total L-dopa Arginine
β P-value β P-value β P-value β P-value
Age 0.38 0.230 0.10 0.358 0.049 0.665 0.062 0.576
Sex –1.34 0.417 –0.12 0.253 –0.096 0.380 –0.087 0.419
BMI SDS 0.075 0.007 0.14 0.204 0.185 0.103 0.183 0.101
PGH_T –0.951 0.004 –0.65 0.028 -
PGH_L –0.669 0.049 –0.156 0.158
PGH_A –0.769 0.012 –0.529 0.049
HOMA-IR 2.087 0.043 0.10 0.360 0.09 0.437 0.098 0.390
Triglyceride 0.073 <0.001 0.07 <0.001 0.07 <0.001 0.067 <0.001
HDL-C –0.175 0.021 0.08 0.571 –0.042 0.741 0.063 0.636

BMI, body mass index; SDS, SD score; PGH-T, peak growth hormone level determined by two stimulation tests; PGH-L, peak growth hormone level stimulated by L-dopa; PGH-A, peak growth hormone level stimulated by arginine; HOMA-IR, homeostatic model assessment-insulin resistance; HDL-C, HDL-cholesterol.

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Univariate analysis showed that the HDL-C level was positively associated with the PGH-T and PGH-A, and was negatively associated with the TG and ALT levels.

In multivariate analyses, PGH-T was a significant predictor of the HDL-C (P=0.002) after adjusting for age, sex, BMI SDS, HOMA-IR, TG, and ALT levels. PGH-A, not PGH-L, also was a significant predictor of the HDL-C (P=0.003).

In univariate analyses, the ALT level was negatively associated with the PGH-T, PGH-L, PGH-A, and HDL-C levels, and was positively associated with the BMI-SDS, HOMA-IR, and TG levels.

In multivariate analyses, each PGH-T and PGH-A, not PGH-L, was a significant predictor of ALT (PGH, P=0.028; PGH-A, P=0.049) levels, after adjusting for age, sex, BMI SDS, HOMA-IR, TG, and HDL-C levels.

Discussion

Similar to previous studies, the present study shows an inverse relationship between the PGH level and BMI SDS in children with GHD. The stimulated PGH level had an independent positive association with the HDL-C level and a negative association with the ALT level, after adjusting for BMI SDS in prepubertal children with GHD.

The PGH level was lower in children in the overweight/obesity group than in those in the normal group, although statistical significance was not reached, possibly due to the small number of overweight and obese participants.

GH is a counter-regulatory hormone of insulin and is involved in glucose metabolism. GH promotes gluconeogenesis and IGF1 production. During fasting and catabolic states, GH induces lipolysis to produce free fatty acid, which switch the metabolism from glucose and protein utilization to lipid utilization to preserve the lean body mass. GH also has anabolic effects on protein synthesis directly and indirectly via IGF1 [13].

In GHD, glucose metabolism varies according to age, puberty, and body composition. Children with GHD have increased insulin sensitivity, which manifests as low glucose and insulin levels, and low HOMA-IR [2830]. In adolescents with GHD who have reached their final height, withdrawal of GH therapy decreased the fasting glucose and insulin levels, and HOMA-IR [19,21]. However, a study of obese children and adolescents showed that PGH level was negatively correlated with HOMA-IR [24].

In adult GHD patients, the insulin level and HOMA-IR were higher compared to the normal controls. Furthermore, the PGH level was inversely correlated with the fasting insulin level and HOMA-IR [15].

The differences in glucose metabolism between GHD adults and children may be due to the physiological changes in insulin resistance with age and body composition. Insulin sensitivity decreases with age and the progression of puberty.

Nonetheless, most GHD patients maintain a normal glucose level irrespective of their insulin level [28].

In the present study, fasting insulin, fasting glucose level, and HOMA-IR were not correlated with the PGH level. These findings may be due to the young age and normal BMI of most study participants. There was no difference in glucose level between the overweight/obesity and normal groups, whereas the HOMA-IR was higher in the overweight/obesity group, but there was no statistical significance (P=0.094).

In GHD and normal adults, a low PGH level was associated with an abnormal lipid profile [15,16,31,32]. Previous studies have reported that GHD children had higher serum TC, LDL-C, and TG levels, and a lower HDL-C level, compared to controls [22,23]. The PGH level was negatively associated with the TC and TG levels in children with short stature, regardless of the presence or absence of GHD [33]. The discontinuation of GH therapy increased the serum TC, LDL-C, and non-HDL-C levels in GHD adolescents [19].

In the present study, the PGH-T level was negatively and positively associated with BMI and the HDL-C levels, respectively. In the partial correlation analysis corrected for BMI and multiple regression analysis, the PGH-T level was also related to the HDL-C level. However, the HDL-C level was lower, but without significance, whereas the LDL-C level was significantly higher in the overweight/obesity group than in the normal group.

Previous studies have also found a significant positive relationship between the PGH and HDL-C levels in normal [32] and GHD adults [15] and in GHD children [24]. Additionally, GH treatment in GHD children and adolescents increased the HDL-C level [23].

These results suggest that the PGH level has a stronger relationship with the HDL-C level than with other lipid parameters.

NAFLD is significantly related to obesity, insulin resistance, and a low IGF1 level [34]. A high prevalence of NAFLD was found in GHD adults [17,18] and adolescents [20]. Obese adults with NAFLD have a lower PGH level compared to healthy controls with a similar BMI [35], additionally, GH treatment is associated with a decreased ALT level in NAFLD adults [17].

In the present study, the ALT level was negatively correlated with the PGH-T level, and the PGH-T level was a significant factor that affected the ALT level in multiple regression analysis, although most participants had a normal ALT level (<40 IU/L).

Few studies have evaluated the presence of NAFLD in GHD children due to the very low prevalence of NAFLD in young children. A recent study reported a negative association between PGH level and ALT level in short children and adolescents without NAFLD [36].

Meanwhile, slightly different results were found when examining the relationship between metabolic parameters with PGH according to the type of stimulants.

PGH-L had a negative association with BMI SDS and ALT level, but not with HDL-C level. PGH-A did not show the relationship with BMI SDS but a positive and negative association with HDL-C and ALT levels, respectively.

Stimulated GH levels by Insulin and clonidine were not evaluated because the number of study was small.

This study had several limitations. First, this was a retrospective cross-sectional cohort study that did not include comparisons with a control group. Second, the number of obese and overweight children with a GHD was small. Additional significant relationships and differences between the groups may be identified in future studies with a large sample size.

Third, pre-pubertal children are usually less prone to metabolic abnormalities. Most of the metabolic parameters in these study participants were within the normal range or showed minimal abnormalities. Further studies are needed that include a large number of children with obvious metabolic abnormalities such as insulin resistance, dyslipidemia and NAFLD.

Fourth, the reliability of GH stimulation tests is uncertain. The PGH level may vary depending on the stimulant used. In the present study, L-dopa and arginine were the most commonly used stimulants, and the highest GH level among the two tests was recorded as the PGH-T level, regardless of the stimulant type used.

Nevertheless, this study provides a meaningful analysis of the relationships between the PGH level and comprehensive metabolic parameters related with insulin resistance, dyslipidemia, and fatty liver, although their levels were within the normal range, in pre-pubertal children with GHD.

The results of this study suggest that among various metabolic parameters, HDL-C and ALT are the most significant metabolic markers related with PGH in children with GHD. However, the relationship between PGH level and HDL-C and ALT was slightly different according to the kind of stimulants.

In conclusion, a low PGH level in pre-pubertal children with GHD is associated with low HDL-C and high ALT, even if they were within normal range, regardless of BMI.

The results of this study suggest that the more severe the GHD, the more likely it is to develop metabolic disturbance such as dyslipidemia and fatty liver. Therefore, patients with GHD with low PGH concentration will need follow-up tests for metabolic abnormalities.

Acknowledgements

Not applicable.

Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Author Contribution

The article is prepared by a single author.

Ethics Approval and Consent to Participate

This study adhered to the Declaration of Helsinki and was approved by the Institutional Review Board (IRB) of SMG-SNU Boramae Medical Center (IRB no. 20-2022-93). The requirement for written informed consent was waived.

References

1.

Cappa M, Loche S. Evaluation of growth disorders in the paediatric clinic. J Endocrinol Invest. 2003; 26:S54-S63

2.

Tassoni P, Cacciari E, Cau M, Colli C, Tosi M, Zucchini S, et al. Variability of growth hormone response to pharmacological and sleep tests performed twice in short children. J Clin Endocrinol Metab. 1990; 71(1):230-234

3.

Devesa J, Lima L, Lois N, Fraga C, Lechuga MJ, Arce V, et al. Reasons for the variability in growth hormone (GH) responses to GHRH challenge: the endogenous hypothalamic-somatotroph rhythm (HSR). Clin Endocrinol. 1989; 30(4):367-377

4.

Loche S, Guzzetti C, Pilia S, Ibba A, Civolani P, Porcu M, et al. Effect of body mass index on the growth hormone response to clonidine stimulation testing in children with short stature. Clin Endocrinol. 2011; 74(6):726-731

5.

Stanley TL, Levitsky LL, Grinspoon SK, Misra M. Effect of body mass index on peak growth hormone response to provocative testing in children with short stature. J Clin Endocrinol Metab. 2009; 94(12):4875-4881

6.

Yang A, Cho SY, Kwak MJ, Kim SJ, Park SW, Jin DK, et al. Impact of BMI on peak growth hormone responses to provocative tests and therapeutic outcome in children with growth hormone deficiency. Sci Rep. 2019; 9(1):16181

7.

Lee NY, Kim SE, Kim S, Ahn MB, Kim SH, Cho WK, et al. Effect of body mass index on peak growth hormone level after growth hormone stimulation test in children with short stature. Ann Pediatr Endocrinol Metab. 2021; 26(3):192-198

8.

Misra M, Bredella MA, Tsai P, Mendes N, Miller KK, Klibanski A. Lower growth hormone and higher cortisol are associated with greater visceral adiposity, intramyocellular lipids, and insulin resistance in overweight girls. Am J Physiol Endocrinol Metab. 2008; 295(2):E385-E392

9.

Barrett J, Maranda L, Nwosu BU. The relationship between subnormal peak-stimulated growth hormone levels and auxological characteristics in obese children. Front Endocrinol. 2014; 5:35

10.

Stanley TL, Feldpausch MN, Murphy CA, Grinspoon SK, Makimura H. Discordance of IGF-1 and GH stimulation testing for altered GH secretion in obesity. Growth Horm IGF Res. 2014; 24(1):10-15

11.

Wilson JR, Utz AL, Devin JK. Effects of gender, body weight, and blood glucose dynamics on the growth hormone response to the glucagon stimulation test in patients with pituitary disease. Growth Horm IGF Res. 2016; 26:24-31

12.

Abawi O, Augustijn D, Hoeks SE, de Rijke YB, van den Akker ELT. Impact of body mass index on growth hormone stimulation tests in children and adolescents: a systematic review and meta-analysis. Crit Rev Clin Lab Sci. 2021; 58(8):576-595

13.

Møller N, Jørgensen JO. Effects of growth hormone on glucose, lipid, and protein metabolism in human subjects. Endocr Rev. 2009; 30(2):152-177

14.

Sperling MA. Traditional and novel aspects of the metabolic actions of growth hormone. Growth Horm IGF Res. 2016; 28:69-75

15.

Cordido F, Garcia-Buela J, Sangiao-Alvarellos S, Martinez T, Vidal O. The decreased growth hormone response to growth hormone releasing hormone in obesity is associated to cardiometabolic risk factors. Mediators Inflamm. 2010; 2010:434562

16.

Lo J, You SM, Wei J, Canavan B, Grinspoon S. Relationship of peak growth hormone to cardiovascular parameters, waist circumference, lipids and glucose in-HIV infected patients and healthy adults. Clin Endocrinol. 2009; 71(6):815-822

17.

Nishizawa H, Iguchi G, Murawaki A, Fukuoka H, Hayashi Y, Kaji H, et al. Nonalcoholic fatty liver disease in adult hypopituitary patients with GH deficiency and the impact of GH replacement therapy. Eur J Endocrinol. 2012; 167(1):67-74

18.

Diniz RD, Souza RM, Salvatori R, Franca A, Gomes-Santos E, Ferrão TO, et al. Liver status in congenital, untreated, isolated GH deficiency. Endocr Connect. 2014; 3(3):132-137

19.

Lee YJ, Choi Y, Yoo HW, Lee YA, Shin CH, Choi HS, et al. Metabolic impacts of discontinuation and resumption of recombinant human growth hormone treatment during the transition period in patients with childhood-onset growth hormone deficiency. Endocrinol Metab. 2022; 37(2):359-368

20.

Kang SJ, Kwon A, Jung MK, Chae HW, Kim S, Koh H, et al. High prevalence of nonalcoholic fatty liver disease among adolescents and young adults with hypopituitarism due to growth hormone deficiency. Endocr Pract. 2021; 27(11):1149-1155

21.

Prodam F, Savastio S, Genoni G, Babu D, Giordano M, Ricotti R, et al. Effects of growth hormone (GH) therapy withdrawal on glucose metabolism in not confirmed GH deficient adolescents at final height. PLoS One. 2014; 9(1)e87157

22.

LaFranchi S, Hanna CE, Illingworth DR. Effect of growth hormone replacement on development of hypothyroidism and hyperlipidemia. J Pediatr. 1985; 106(4):588-593

23.

Chen M, Gan D, Luo Y, Rampersad S, Xu L, Yang S, et al. Effect of recombinant human growth hormone therapy on blood lipid and carotid intima-media thickness in children with growth hormone deficiency. Pediatr Res. 2018; 83(5):954-960

24.

Liang S, Zhang D, Qi J, Song X, Xue J. Reduced peak stimulated growth hormone is associated with hyperuricemia in obese children and adolescents. Sci Rep. 2018; 8(1):7931

25.

Kim JH, Yun S, Hwang SS, Shim JO, Chae HW, Lee YJ, et al. The 2017 Korean National Growth Charts for children and adolescents: development, improvement, and prospects. Korean J Pediatr. 2018; 61(5):135-149

26.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972; 18(6):499-502

27.

Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985; 28(7):412-419

28.

Ciresi A, Giordano C. Glucose metabolism in children with growth hormone deficiency. Front Endocrinol. 2018; 9:321

29.

Cañete R, Valle M, Martos R, Sánchez-Carrión A, Cañete MD, van Donkelaar EL. Short-term effects of GH treatment on coagulation, fibrinolysis, inflammation biomarkers, and insulin resistance status in prepubertal children with GH deficiency. Eur J Endocrinol. 2012; 167(2):255-260

30.

Husbands S, Ong KK, Gilbert J, Wass JA, Dunger DB. Increased insulin sensitivity in young, growth hormone deficient children. Clin Endocrinol. 2001; 55(1):87-92

31.

Vahl N, Klausen I, Christiansen JS, Jørgensen JO. Growth hormone (GH) status is an independent determinant of serum levels of cholesterol and triglycerides in healthy adults. Clin Endocrinol. 1999; 51(3):309-316

32.

Carmichael JD, Danoff A, Milani D, Roubenoff R, Lesser ML, Livote E, et al. GH peak response to GHRH-arginine: relationship to insulin resistance and other cardiovascular risk factors in a population of adults aged 50-90. Clin Endocrinol. 2006; 65(2):169-177

33.

Zhao Q, Chu Y, Pan Y, Zhang M, Ban B. Association between triglyceride glucose index and peak growth hormone in children with short stature. Sci Rep. 2021; 11(1):1969

34.

Dichtel LE, Corey KE, Misdraji J, Bredella MA, Schorr M, Osganian SA, et al. The association between IGF-1 levels and the histologic severity of nonalcoholic fatty liver disease. Clin Transl Gastroenterol. 2017; 8(1)e217

35.

Dichtel LE, Corey KE, Haines MS, Chicote ML, Kimball A, Colling C, et al. The GH/IGF-1 axis is associated with intrahepatic lipid content and hepatocellular damage in overweight/obesity. J Clin Endocrinol Metab. 2022; 107(9):e3624-3632

36.

Ji B, Zhang M, Zhao Q, Chu Y, Li Y, Pan H, et al. Association between alanine aminotransferase and growth hormone: a retrospective cohort study of short children and adolescents. Biomed Res Int. 2019; 2019:5939372