The availability of combined antiretroviral therapy has significantly reduced the number of new HIV infections and the associated mortality, and HIV infection has become a chronic disease with long-term survival. In Korea, more than 1,000 new HIV infections have been registered annually since 2013. After peaking at 1,223 in 2019, the number of new infections decreased between 2020 and 2023. In 2023, the majority of newly HIV-infected people were men, and the proportions of young people under 40 years, homosexual contacts and foreigners increased. Acquired immunodeficiency syndrome (AIDS)-related deaths from opportunistic infections associated with immunosuppression and AIDS-defining cancers are gradually decreasing, whereas non-AIDS defining comorbidities such as non-AIDS defining cancers, cardiovascular disease and metabolic complications are emerging as major causes of death. Since the introduction of zidovudine, approximately 30 antiretroviral drugs have been approved for the treatment of HIV infection. Early and continuous antiretroviral treatment for all people living with HIV is an effective strategy for maintaining viral suppression and preventing transmission of HIV infection. In conclusion, achieving the 95–95–95 target among those living with HIV in Korea requires multifaceted efforts to improve early diagnosis, early and proper treatment of HIV infection including the management of chronic diseases, and adherence to antiretroviral therapy.
Citations
, Sang-Jung Kim, Kyoung Hwa Yoo, Kyung Han Lee, Gi Tark Noh, Won Seok Heo The incidence of tuberculosis (TB) had gradually been declining all over the world, but in recent years, TB has been increasing due to the spread of the human immunodeficiency virus (HIV). When immune-suppression status deteriorates further, extrapulmonary TB generally appears more often. Abdominal TB is one type of extra-pulmonary TB, which may involve the gastrointestinal tract, peritoneum, lymph nodes or solid viscera. We encountered a case who had initially been diagnosed as having abdominal TB, had progressed to acute respiratory distress syndrome and was eventually confirmed as having developed acquired immune deficiency syndrome. In cases of coinfection of TB and HIV, it is reported that immunological responses become poor and complications with higher morbidity frequently occur. Therefore, the Korean guidelines for TB should be revised to ensure whether HIV infection exists in TB patients.
To compare the diagnostic performance of a high-resolution picture archiving and communications system(PACS) workstation directly interfaced with computed tomography(CT) with hard-copy printouts and to compare the detection rate according to slice thickness in hepatocellular carcinomas(HCCs).
Forty-six patients with 118HCCs underwent two-phase multi-detector row helical CT imaging of the entire liver after contrast administration. Late arterial phase images were obtained serially during a single breast-hold, and portal venous-phase images were then obtained. In soft-copy, images taken in each phase were reconstructed by 3mm and 7mm in thickness. Soft-copy readouts on a workstation in PACS and hard-copy printouts were independently compared for the presence of HCC by two radiologists unaware of the possible presence of tumors, and for each phase the detection rate was determined in 7mm thickness. The detection rate of HCC displayed on a workstation was analyzed in 3mm and 7mm thickness for each phase.
No significant differences in observer performance were observed between laserprinted hard copies and CT images displayed on a workstation(p>0.05). But the detection rate of HCC displayed on workstation was higher in 3mm thickness(p<0.05).
The diagnostic performance of CT hard copies is acceptable and comparable to a high-resolution PACS workstation in hepatocellular carcinomas and the detection rate of HCC on PACS workstation is significantly higher in thin slice thickness.
, Eun-Sun Yoo Stable gene transfer to human pluripotent hematopoietic stem cells is an attractive strategy for the curative treatment of many genetic hematologic disorders. In clinical tral, the level of gene transfer to this cell population have generally been low.
In this study we have evaluated the efficiency of gene transfer to human umbilical cord blood(UCB) CD34+cells using vesicular stomatitis virus glycoprotein G(VSV-G) pseudotyped HIV-1 vector.
High titers of replication-defective VSV-G pseudotyped HIV-1 based vector encoding the enhanced yellow fluorescent protein were produced by transient transfection. Human CD34+cells purified from UCB were incubated with pseudotyped HIV supernatants for 24-48 hours. The transduction efficiency were measured by marker gene expression under the microscopy and flow cytometry.
Transduction rates into CD34+ were low at 0 and 24 hours, reflecting 4.7±2.4% at 24 hours, they were increased to 5.7±2.7% at 48 hours.
We demonstrate efficient transduction of purified human UCB CD34+ by HIV vectors pseudotyped with VSV-G. The results extend the lentiviral vector to clinical gene therapy using human pluripotent hematopoietic stem cells.
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